Shade's tour speech at the end mentions Icicle, Sand, and Damage. In the comics, Icicle is the alias that Cameron eventually takes on. Sand is the previously mentioned nephew of the original JSA member Wesley Dodds, aka Sandman. Sand's mask is shown when Shade talks about other legacies of heroism. Damage is the son of the Golden Age Atom, who has also been previously mentioned as a member of the original JSA. The Seven Soldiers' nemesis the Nebula Man is also mentioned.
In the comics, Flash and Shade were arch-enemies. Here they eventually became friends, as noted when Flash calls Shade "old friend".
On the museum tour signs direct visitors to other parts of the building including the Wildcat Wing, Dr. Mid-Nite's Interactive Goggle Experience, and Dragon Queen's Lab in the basement.
At 39:08 a quote can be seen on the wall by Green Lantern about darkness not being able to stand up against the light. Poignantly, the plaque and the JSA meeting table stand in the light, and the tour guide, The Shade, stands like a shadow next to them.
There are two teens on the tour who are fans of the JSA. The teen boy wears a Stripesy shirt and the teen girl wears a shirt with a star on it.