First main cast reunion since the show ended in 2015, and the first time Donald Glover has reprised the role of 'Troy' since his departure from the show during season 5. Yvette Nicole Brown (Shirley) also left the show at the end of season 5, but made two guest appearances in the final season.
Walton Goggins (Mr. Stone) wasn't available due to a scheduling conflict, which led to Pedro Pascal taking over his role.
During the reading, Gillian Jacobs (Britta) revealed she still has one of the sperm cylinder props from the original episode.
Main cast member Jim Rash (Dean Pelton) did not appear in the original version of "Cooperative Polygraphy", but fills in during the table read as Mara the polygraph technician and other minor characters.
Regular cast members John Oliver (Ian Duncan), Jonathan Banks (Buzz Hickey), Paget Brewster (Frankie Dart) and Keith David (Elroy Patashnik) didn't take part in the table read. Jonathan Banks and John Oliver were both part of the cast during season five (in which "Cooperative Polygraphy" aired), but didn't appear in the original episode, and Paget Brewster and Keith David only joined the cast in season six.