In closeup, Dr. Moon (in Donna's living room) picks up his briefcase with his right hand, with the "CAL" label facing inward, towards himself. In the wide shot, he is suddenly holding the briefcase with the label facing outward.
The Doctor stresses that they must not touch shadows, because any of them could potentially be infected, yet he and River cross Anita's shadow to put her helmet on after knowing that she is infected.
Around 18 minutes, the girl in the playground has blood on her knee but doesn't fall over until later around 25 minutes.