Originally, Will Arnett was cast as the voice of K.I.T.T. However, after he had finished recording his lines to everyone's satisfaction, a conflict of interest was raised. His is the distinctive voice heard on General Motors car and truck commercials, but K.I.T.T. is a Ford vehicle. As a result, Val Kilmer was cast as K.I.T.T.'s voice and all of the car's dialog was re-recorded.
In the beginning, as the thieves are going through the garage, you can see many of the parts from a third generation Firebird, including the outline of an opened hood, a Tune Port Injected engine, as well as the dash of the Knight Industries Two Thousand.
Mike Traceur is initially living in Las Vegas, which is where Michael Knight had been a cop before being recruited into FLAG in the original show.
In the scene where Sarah explains KITT's fuel technology, Mike asks "Why you gotta hate on Nebraska?" This is an inside joke to Justin Bruening, as he is from St. Helena, Nebraska.
In this series K.I.T.T stands for Knight Industries Three Thousand, not to be confused with the original K.I.T.T. (Knight Industries Two Thousand).