No mention is made of the Lizard's alter-ego, Dr. Curt Conners, which is unusual as his dual nature is a key part of his character in the original comic book stories.
The original headquarters of the Fantastic Four, the Baxter Building, can be seen in the background of one of the shots of Spider-Man swinging over New York. Spider-Man also swings past a billboard advertising "The Incredible Hulk" live action series, which was still being produced at the same time as this series.
A poster for the pop band B-52's can be seen in the background during the subway car scene.