Beginning where Hostel ended, Paxton (Jay Hernandez), now in seclusion with his girlfriend Stephanie (Jordan Ladd), disregards her pleas to report his friends' deaths. In the morning, Stephanie discovers Paxton's decapitated body in the kitchen being licked by a kitten. In Slovakia, a mysterious package is delivered to Sasha (Milan Kako), the owner of the entire money-for-murder business that begins in the hostel. In Italy, art students Beth (Lauren German), Whitney (Bijou Phillips) and Lorna (Heather Matarazzo) are convinced by Axelle (Vera Jordanova), a model they were sketching, to join her on a spa vacation. The party travels to a small Slovak village and checks into the local hostel and retires to their rooms, allowing the desk clerk time to upload their passport photos to an online auction website, where American businessman Todd (Richard Burgi) bids on and wins Whitney and Beth for himself and his best friend Stuart (Roger Bart).
Later that night at the village's "Harvest Festival," as the girls socialize with the locals, Lorna discovers that Beth previously inherited a vast fortune from her mother. As Todd and Stuart observe the girls from across the river, Stuart approaches Beth and the two share a friendly if awkward conversation before separating. An intoxicated Lorna leaves with Roman, a charismatic local, on a boat ride to a secluded shore, where Lorna is kidnapped by Roman and two accomplices. Beth and Whitney leave the party, while Axelle volunteers to wait for Lorna. The next morning the three girls, assuming Lorna is enjoying Roman's company, travel to the local spa. Meanwhile, a naked Lorna is shackled upside-down in a large room, where a woman (Monika Malacova) enters, named Ms. Bathory in reference to the infamous Hungarian serial killer. She undresses and lies beneath Lorna in a candlelit recess and proceeds to execute her by sexually sadistic torture murder. After ripping into Lorna's body with a scythe and bathing in her blood, she slits Lorna's throat with a sickle. At the spa, Beth awakes to find herself alone and her belongings stolen. As she looks for her friends, she notices two men approaching her, and escapes into the forest, where she is ambushed by the "Bubblegum Gang" before being rescued by Axelle and Sasha. After Beth is taken to Sasha's mansion, she realizes that Sasha and Axelle are responsible for Whitney and Lorna's disappearances after seeing the men who had tried to kidnap her at the spa coming up the stairs of Sasha's home to her room. She quickly tries to hide and uncovers a room filled with severed heads, including Paxton's, before being kidnapped.
Deep inside the factory, a sobbing Whitney is strapped to a chair in a cell while an old woman applies makeup to her face. Whitney bites the woman's face and escapes, only to be captured by the guards. Whitney is then taken to Todd's cell and strapped to a chair while he teases her with a circular saw. Overwhelmed with excitement, Todd accidentally catches her face, maiming her, which sufficiently disturbs him such that he no longer wants to kill her. As he leaves the room, the guard explains that he must kill her if he wants to leave; when he refuses and continues to make his way out, the guards set dogs on him to kill him for violating his contract. The guards bandage Whitney and show her photograph to other clients in adjoining cells, offering her at a discount. Stuart, now torturing Beth, accepts the offer and kills Whitney after he sees what the dogs had done to Todd, assuming that Whitney was at fault. As he returns to Beth, she tricks him into releasing her from the chair, she fights him off and chains Stuart to the chair. Beth grills Stuart for the code to the cell, when he refuses to tell her, she sticks a needle in his ear, Stuart tells Beth the wrong code and she inadvertently summons Sasha and his guards to the room. Beth offers to buy her freedom with part of her inheritance. When Sasha explains to her that she must kill somebody to leave, Beth cuts Stuart's penis off and tosses it to the guard dogs who eat it; Beth then orders for Stuart to be left to bleed to death. Per the standard contract, Beth is given an Elite Hunting tattoo. In the closing sequence, Axelle is lured from the village festival into the woods by the Bubblegum Gang, where Beth surprises and beheads her, allowing the gang to play soccer with her head.
(Source: WikiPedia)