The film was originally intended to be filmed in the Australian Outback but was changed to Mexico.
This is the first live-action movie trilogy based on a video game.
The accompanying music for the first theatrical trailer is The Crystal Method's "I Know It's You". Milla Jovovich provides the vocals/wailing for the song, which was released three years earlier.
Prints were shipped to some theaters under the fake title "Raccoon City" - which is the name of the city featured in many of the video games.
In the Tokyo scene the sign next to 'Tokyo Metro' reads 'Zatoichi Square' an obvious nod to the fictional Japanese film and television character.
WILHELM SCREAM: The man who is using the flamethrower when he gets knocked off the top of the truck by the infected ravens.
The film was not screened for critics, most likely due to the overwhelmingly-negative reviews from critics for the first two films.
For the crow attack scene, only two crows were used for the entire sequence. The rest of the crows were entered with special effects.
There's a sneak preview of the next Resident Evil movie Resident Evil: Degeneration (2008) in the bonus material of the DVD.
This is the first Resident Evil movie not to be released onto the VHS format, all the other movies were.
Claire Redfield's role in the movie is different than in the games. She leads a convoy of survivors across the dead desert, but in the games, she on a mission to find her lost brother Chris Redfield.
The crows sitting on power lines and attacking in large numbers pays homage to Alfred Hitchcock's movie The Birds (1963).
The Blu-Ray release was the first Blu-ray movie that offered the 'picture in picture' feature.
Raccoon City is clearly Detroit, Michigan from the graphic about 6-7 minutes into the film. The Detroit river and Belle Isle are clearly discernible, as is the half moon shaped industrial park to the North (towards the left in the film).
Sienna Guillory was offered the chance to reprise her role as Jill Valentine from Resident Evil: Apocalypse (2004), but had to turn it down due to scheduling conflicts with Eragon (2006) and the character was then written out of this sequel.