The position of Kinsey's fingers changes when he tells the interviewer a list of childhood ailments.
When everyone is sitting around listening to music with a group, Clyde and Mac share an awkward glance, but they look the wrong way. In the wide shot, Mac is to Clyde's left, but the closeups have Clyde looking to his right and Mac looking to her left.
During the credits, the producers thank the "University of Indiana" when it is actually "Indiana University" of which Alfred Kinsey was a part. The university notified director Bill Condon of the mistake. Condon gave his word that it would be taken care of when the film went on general release, but the mistake remains.
The scene where Kinsey's mother, Sara, is buried, an outdoor funeral prayer is heard and family and friends are visiting Alfred Sr. (Seguine) at his home is incorrect: Kinsey's mother didn't die before Alfred Sr.; his parents were divorced in 1931 when Alfred Jr. (the titular Kinsey) was about 37 and before Alfred Jr. even started his research for his first "Sexual Behavior" book. Also, the funeral prayer sounds more like a Catholic one and Kinsey's family were Methodists.
When Kinsey and his wife are in the woods in New England one afternoon, they hear a whippoorwill, but the whippoorwill is a nocturnal bird.
When young Kinsey argues with his father in the general store, the actor's prosthetic nose is extremely visible, several shades darker than his skin with wrinkly edges
During Kinsey's lecture a phonograph is shown playing a classical recording. The music is heard but the tone arm's position is shown not playing the record.
The newspaper headlines about Kinsey's first book in the late 1940s are pasted onto current newspapers. Just above one of the headlines we see a story about the New York Mets and the Houston Astros. These teams did not exist in the late 1940s.
In a flashback to Kinsey's childhood, his father preaches a sermon against the zippered fly. Kinsey is shown as a boy of 10, yet in reality he would have been 23 when the device was patented, 31 when it was named the "zipper," and about 40 when it began to be used on pants.
The Boy Scouts (Kinsey and his friend) are wearing the World Crest emblem which did not exist in the late teens when this scene supposedly took place.
The dial tone on one phone used in the movie at the time portrayed had a sound not used by telephony until much later. It was ooooo instead of the earlier arrrr as would have been accurate.
The chronology of the film suggests that the publication of a "new book" by Alfred Kinsey, The Origin of Higher Categories in Cynips, the appointment of Herman Wells as the president of Indiana University, and the showing of the "hygiene" film Know for Sure (1941) all occurred within a relatively short period of time. However, Kinsey published the book in question in 1935, Wells was not appointed as president until 1937 (interim) or 1938, and the film was not released until 1941.
Kinsey is talking with reporters upon arriving in New York and his dialogue does not match up with the shot of him.
About 25 minutes into film someone plays 'Frederic Chopin' Etude #1 in A flat. The notes do not match what is being played. Only part of the keyboard is seen but the right hand seems to be playing an octave lower than the notes.