- At the end of the credits, there are audition tapes of Alexa Vega (Carmen), and Daryl Sabara (Juni) for the first Spy Kids movie.
- The HD/3-D process is the same used by director 'James Cameron' for his IMAX feature Ghosts of the Abyss (2003), although the projection is different. Cameron used polarized projection only viewable in special venues. Rodriguez used the traditional anaglyph (notable for its scarlet red and cyan blue lensed glasses) projection. The cameras used for the film consisted of two custom-designed Sony HDC-950 cameras (HDCAM) which have had their image sensors separated from the main body of the camera and rehoused 70mm apart - the same distance between a pair of human eyes. This also allowed director Robert Rodriguez to view immediate 3D playback on-set.
- Sylvester Stallone said in an interview that he asked his kids about the previous Spy Kids movies and if he should accept the role in the third installment.
- When Juni and Grandpa emerge from the game, Grandpa says that the armrests on his wheelchair are covered in rich Corinthian leather. This is a reference to actor Ricardo Montalban's Chrysler Cordoba television commercials in the late '70's ("The seats are upholstered in rich Corinthian leather").
- Prior to the film's release, it was announced that this would be the final Spy Kids movie, as the young actors, in particular Alexa Vega were literally growing out of the role.
- When Juni is entering the next level of the video game, he comes across a place named "Rebel Rockets" the names of director Robert Rodriguez's two sons.
- Cameo: [Elijah Wood] 'Robert Rodriguez (I) wrote the role of 'The Guy' with him in mind. Wood had previously starred in Rodriguez's The Faculty (1998) shortly before finding out about The Lord of the Rings: The Fellowship of the Ring (2001). The suit that Wood wore was originally one for the character Juni, but they spray-painted it silver and Cellotaped it to him as it barely fit. In the shot where Wood is kneeling down, talking to the others, he is actually looking at the ground to give the impression that he is towering over everybody.
- Due to filming schedules, many of the actors playing characters in the final scene were never on the set at the same time and were put together digitally.
- When Juni walks into OSS, shortly after discovering what happens in “Game Over”, you see Rocket Rodriguez, Robert's oldest son.
- In Italy, for the theatrical release, the film was renamed simply "Missione 3D: Game Over" ("3D Mission: Game Over"), without any references in the trailers, or anywhere else, of it being a part of the "Spy Kids" trilogy.
- Near the end of the film, Grandpa warns to be careful of his wheelchair because it's "Corinthian leather." This is an in-joke referring to a commercial Ricardo Montalban did for the 1975 Chrysler Cordoba in which he praises the Corinthian leather of that car.
>>> WARNING: Here Be Spoilers <<<
Trivia items below here contain information that may give away important plot points. You may not want to read any further if you've not already seen this title.
- SPOILER: George Clooney's scenes were shot in one afternoon in his own living room. He shot the scenes in a suit jacket, shirt, tie and pajama pants.
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