- A "National Geographic" film crew is taken hostage by an insane hunter, who forces them along on his quest to capture the world's largest - and deadliest - snake.
- When a documentary crew traveling through the Amazon jungle, picks up a stranded man, they are unaware of the trouble that will occur. This stranger's hobby is to capture the giant Anaconda snake, and plans to continue targeting it on their boat, by any means necessary.—FilmFanUk
- In search of the elusive Shirishama tribe, the bold director, Terri Flores, leads an equally ambitious team of six documentarians deep into the Brazilian rain forest. Before long, while sailing the meandering Amazon River, a chance encounter with the stranded adventurer, Paul Serone, who claims that he has evidence of the mysterious indigenous tribe's existence, will renew the group's hopes. However, this is the habitat of a monstrous scaly predator, and Paul seems to know more than he's letting on. Now, slow and agonising death awaits the unsuspecting invaders. Is there an escape from the ever-tightening coils of the mighty, twelve-metre anaconda?—Nick Riganas
- A film crew headed by anthropologist Steve Cale and director Terri Flores journey through a river to find a mysterious Indian tribe and shoot a documentary on them. They save a guy called Paul Sarone from a sinking boat. He offers to help in their search for the tribe, but he is actually a snake-hunter looking for legendary anaconda snake, and when Cale is bitten by some tropical insect, Sarone takes over the boat.—Anonymous
- A "National Geographic" crew heads down the Amazon in a jungle boat to make a documentary on a long-lost tribe of natives. En route, they rescue a hunter from his sinking boat and take him on board. After the ship's captain is attacked by a poisonous insect and goes comatose, the hunter commandeers the boat, takes the crew hostage, and drags them along on his insane quest to capture a giant anaconda.—Scarface
- Shooting a documentary about a long-lost Indian tribe on the Amazon River, director Terri Flores (Lopez) and members of her crew, including cameraman Danny Rich (Ice Cube) and Professor Steven Cale (Stoltz), come across stranded snake hunter Paul Sarone (Voight) and help him, believing he knows how to find the tribe they're searching for, but Sarone acts strangely and the crew suspect something. When Cale is bitten by a poisonous wasp which entered his mouth (leaving him unconscious for nearly the rest of the film), Sarone takes command of the boat...and the crew.
They are then forced to help him achieve his true objective; hunting down and capturing a record-breaking Green Anaconda he had been tracking. During the process, several members of the crew are killed before the snake itself is shot dead and Sarone incapacitated by the newly awakened Cale.
Later on, Terri and Danny, two of the surviving members of the crew, are captured when Sarone catches up to them. He dumps a bucket of animal blood on them and then uses them as bait in an attempt to capture a second, larger Anaconda.
The anaconda, Terri and Danny are caught in a net by Sarone, but escape when the snake breaks free. The survivors cut their bonds and break free as Sarone himself is killed by his quarry. The snake becomes trapped in the smoke stack, which Danny ignites and it seems to have died. As Terri and Danny recuperate on a nearby dock and the snake appears one final time. Danny beats the anaconda with an axe until it is finally slain.
Afterwards, the survivors of the expedition reunite on the ship, and as they float down the river, accidentally locate the natives they were originally searching for. They realize Sarone was right and begin filming their documentary as the movie ends.
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