- Depending on the condition and color saturation of the print, the Joker's hair can appear orange rather than green. This was evident on the VHS releases of the film. The same thing would sometimes happen in the TV series (particularly during fades to black), but the mistake was more evident and consistent in the film version. The Blu ray release color saturation corrects this and the Joker's hair appears green as it should.
- Some American TV prints omit the sequence showing Batman and Robin driving the Batmobile down a boat dock to reach the Batboat (including a gag showing them using another set of batpoles to get down to sea level).
- Some TV prints cut Penguin's final line in the scene in which he dehydrates the pirates ("Every one of the them has a mother!").
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